Nextseq 550 sequencing system. NextSeq 550 Applications: Gene Expression Profiling; Exome Sequencin...

NextSeq 1000 & 2000 Sequencing Systems; TruSigh

Figure 1: NextSeq 500 System Sample-to-Answer WGS Sequencing Workflow. The NextSeq 500 System’s simple workflow delivers highly accurate sequencing data. Data analysis includes alignment and variant calling. A Fast, Efficient WGS Workflow The NextSeq 500 System WGS workflow offers a simple, fast, end-to-end solution for characterizationExome sequencing analyzes the protein-coding region of the genome, as a cost-effective alternative to whole-genome sequencing. Exome sequencing with the NextSeq 550 System offers a simple and reliable method to identify coding variants, successfully sequencing even the most difficult genomic regions. Sequence 3–12 exomes per NextSeq run. NextSeq 550Dx has a diagnostic mode and a research mode, enabling its use in clinical research and for developing in vitro diagnostic assays. Illumina said that the setup of workflows for different applications is "fully integrated" into the instrument's software, and that it "offers fully-integrated onboard analysis software with modular ...20074933. Provides kitted reagents for 100 cycles of sequencing, plus dual- indexing support (up to 100M single reads). Includes: NextSeq 1000/2000 Reagent Cartridge (100 cycles), NextSeq 1000/2000 P1 Flow Cell, and RSB with Tween 20. This …NextSeq 550 System Sequencing Performance Parameters. *Install specifications based on Illumina PhiX control library at supported cluster densities (between 129 and 165 k/mm² clusters passing filter). Actual performance parameters may vary based on sample type, sample quality, and clusters passing filter. All NextSeq 550 kits are paired-end ... The NextSeq 550 System is a good fit for customers who already own that system and want to expand capacity with existing workflows. The NextSeq 550 System also provides labs with microarray scanning capabilities. How is the integrated dry cartridge different from the reagents of the MiSeq ™ or NextSeq 550 Systems?The NextSeq 550 System offers various throughput levels, providing users with optimal operational efficiency. The NextSeq 550 System also delivers a one-day turnaround for numerous popular sequencing applications. With this instrument, users can sequence a broad range of samples per run: • 1-12 exomes • 1-16 transcriptomesSystem Instrument NextSeq 500/550 15046626 SY- 415- 1002 NextSeq® 550 Sequencing System Illumina NextSeq 550 Sequencing System is for Research Use Only and is an integrated system for automated generation of DNA clonal clusters by bridge amplification, sequencing, primary analysis, and array scanning. System includes embedded touchscreenThe NextSeq 550Dx makes it easy to meet your needs with flexible read lengths and multiple output configurations. Access a growing pipeline of assays with shortened …Sequencing Consumablestopic. ... NextSeq System Site Prep Guide (15045113) ... Illumina Subject: Information about preparing the lab for the NextSeq 500 and NextSeq ...standard reagent kits, NextSeq Systems, and NovaSeq 6000 with v1.5 reagent kits. For the HiSeq X, HiSeq 4000, and HiSeq 3000 Systems, the Index 2 sequencing primer is part of HP14. HP14 is an indexing primer mix that contains primers for both index reads. Figure 3 Dual-Indexed Sequencing on a Paired-End Flow Cell (Reverse Complement) 1.NextSeq 550 System Sequencing Performance Parameters. *Install specifications based on Illumina PhiX control library at supported cluster densities (between 129 and 165 k/mm² clusters passing filter). Actual performance parameters may vary based on sample type, sample quality, and clusters passing filter. All NextSeq 550 kits are paired-end ... MiSeq Sequencing System (illumina) USD. Used. Illumina NextSeq 500 DNA Sequencer. USD. Used. ... 2019 ILLUMINA NEXTSEQ 550 RNA/DNA SEQUENCER. Please InquireNextSeq 1000 & 2000 Sequencing Systems; TruSight Oncology Product Family; All Popular Products. ... MiniSeq, MiSeq, NextSeq 550: MiniSeq, MiSeq, NextSeq 1000, NextSeq 2000, NextSeq 550: Example Workflow . Prepare Library with: Respiratory Pathogen ID/AMR Enrichment Kit . Sequence Efficiently with:same high standards of data quality enjoyed by NextSeq 550 System users today. High-quality data and lower run costs with enhanced reagent chemistry Building on decades of expertise, the NextSeq 1000 and ... and NextSeq 2000 Sequencing Systems are ideal for wide-ranging applications (Table 2) across oncology, genetic disease, reproductive ...The NextSeq 550 System combines tried-and-true instrument technologies and tunable output with sequencing and array capabilities. Users can run transcriptome, targeted resequencing, genotyping, and other applications all on one platform. View Video Order Online. The NextSeq 500 Sequencing System has been discontinued.This document applies to all staff that operate the Illumina NextSeq 500/550 and supervisors that oversee these operations. ... nextseq-500-system-guide-15046563- ...NextSeq 500/550 Sequencing System and the NextSeq 550Dx Sequencing System (Research Mode). This software release is only compatible with Windows 10 operating system and is not backwards compatible with Windows 7. For more information on the NextSeq 500, NextSeq 550, or NextSeq 550Dx, see the following guides available on www.illumina.comFigure 1: NextSeq 500 System Sample-to-Answer WGS Sequencing Workflow. The NextSeq 500 System’s simple workflow delivers highly accurate sequencing data. Data analysis includes alignment and variant calling. A Fast, Efficient WGS Workflow The NextSeq 500 System WGS workflow offers a simple, fast, end-to-end solution for characterizationTunable, robust sequencing performance The NextSeq 550 System streamlines the exome sequenc - ing workflow. It takes less than 10 minutes to load and initiate the system. Sequencing is completed in as few as 18 hours for up to 16 samples using the high-output mode and paired-end 75-bp read lengths. Two flow cell config -NextSeq 500/550 v2.5 sequencing reagent kits deliver the power of high-throughput sequencing on a benchtop system with increased stability and robustness. These kits offer: New v2.5 flow cell provides greater stability and robustness. Continued use of proven v2 reagent and buffer cartridges. Multiple options for both sequencing output and read ... Order the NextSeq 550 System. The flexible NextSeq 550 System offers a seamless transition between high-throughput sequencing and array scanning. Accelerate your cytogenomics research for constitutional disorders and cancer. Benefit from the sequencing power of the system, then confirm structural variants with complementary array technology. A fast and intuitive workflow to maximize your time and resources. The NextSeq 1000 and NextSeq 2000 Systems support a wide range of methods such as exome sequencing, target enrichment, single-cell profiling, transcriptome sequencing, and more. With DRAGEN onboard, the systems offer an intuitive workflow with load-and-go ease and push-button ...NextSeq 500 System Guide in Spanish. PDF (1 MB) Feb 22, 2022. Instructions for using the NextSeq 500 sequencing system.No match for its versatility and sequencing data. Rating: 4.3. Application Area: DNA/ RNA sequencing. " High-throughput sequencing system having capacity to sequence exomes or whole genomes in one go. ". Review date: 08 Feb 2021 | NextSeq™ 550Dx Next-Generation Sequencing System.NextSeq 500 and 550 System Denature and Dilute Libraries Guide. Instructions for preparing PhiX and denaturing and diluting libraries for sequencing on the NextSeq 500 and 550 Systems. View Options.NextSeq System Suite Installer Version 4.0 Upgrade Download: Technical Note: Illumina Sequencing Platforms Brochure Download: Brochure: 14 MB: Apr 17, 2023: NextSeq 550 amplicon sequencing solution Download: Application Note < 1 MB: Jan 29, 2021: NextSeq 550 exome sequencing solution Download: Application Note < 1 MB: Jan 29, 2021: NextSeq 550 ... Deciphering the Role of Long Non-Coding RNA in Cancer. Researchers are using RNA-Seq to reveal how lncRNAs could be used to identify, measure, and treat cancer. See how scientists are using the NextSeq 550 System and access related product literature, videos, and webinars.NextSeq 500/550 v2.5 sequencing reagent kits deliver the power of high-throughput sequencing on a benchtop system with increased stability and robustness. These kits offer: New v2.5 flow cell provides greater stability and robustness. Continued use of proven v2 reagent and buffer cartridges. Multiple options for both sequencing output and read ... NextSeq 550 System: Samples per run (high output): 16 (8 DNA + 8 RNA), 10 if DNA only, 16 if RNA only: 2 × 101 bp (max recommended) ... NextSeq 500/550 v2.5 Kits. These sequencing reagent kits offer increased stability and robustness over the v2 kit version. TruSight Oncology 500.The NextSeq 550 System combines tried-and-true instrument technologies and tunable output with sequencing and array capabilities. Offers a validated high-throughput …This course shows you how to get started using the Local Run Manager v2 software to set up your sequencing runs and view analysis results. Local Run Manager v2 is available for MiniSeq instruments using MiniSeq Control Software v2 or later, NextSeq 500/550 instruments using NextSeq Control Software v4 and later, and MiSeq instruments using ...in the v2 reagent cartridge. For more information, see the NextSeq 500/550 Kit v2 Reference Guide (document # 15058065). Added Sequencing Consumables section that lists kit versions, compatible NCS versions, and the name and part number of the associated kit reference guide. Updated user-supplied consumables to specify uses of NaOCl for Illumina NextSeq 550. The NextSeq 550 is a mid-range output sequencer. Multiple run options are offered, with outputs ranging from 16 Gb to 120 Gb and read ...A fast and intuitive workflow to maximize your time and resources. The NextSeq 1000 and NextSeq 2000 Systems support a wide range of methods such as exome sequencing, target enrichment, single-cell profiling, transcriptome sequencing, and more. With DRAGEN onboard, the systems offer an intuitive workflow with load-and-go ease and push-button ...NextSeq 550Dx has a diagnostic mode and a research mode, enabling its use in clinical research and for developing in vitro diagnostic assays. Illumina said that the setup of workflows for different applications is "fully integrated" into the instrument's software, and that it "offers fully-integrated onboard analysis software with modular ...The NextSeq 550 System offers various throughput levels, providing users with optimal operational efficiency. The NextSeq 550 System also delivers a one-day turnaround for numerous popular sequencing applications. With this instrument, users can sequence a broad range of samples per run: • 1-12 exomes • 1-16 transcriptomesThe NextSeq 1000 and 2000 systems leverage proven sequencing by synthesis (SBS) technology to to deliver highly accurate data and robust performance. SBS is the same proven technology used by all Illumina sequencing platforms. The technology supports massively parallel sequencing using a proprietary fluorescently labeled reversible terminator ...Order the NextSeq 550 System. The flexible NextSeq 550 System offers a seamless transition between high-throughput sequencing and array scanning. Accelerate your cytogenomics research for constitutional disorders and cancer. Benefit from the sequencing power of the system, then confirm structural variants with complementary array technology.The NextSeq 550 System combines high-quality array scanning with the proven and robust NextSeq 500 sequencing system. By leveraging array scanning on the NextSeq 550 System, researchers have instant access to a powerful, complementary technology to confirm copy number variants detected through sequencing. Download Data Sheet.data quality enjoyed by NextSeq 550 and MiSeq™ System users. Figure 1: The NextSeq 2000 Sequencing System —The NextSeq 2000 System offers innovative design features, advanced chemistry, simplified bioinformatics, and an intuitive workflow that enable the widest range of applications and flexibility of scale on a benchtop sequencing system.data quality enjoyed by NextSeq 550 and MiSeq™ System users. Figure 1: The NextSeq 2000 Sequencing System —The NextSeq 2000 System offers innovative design features, advanced chemistry, simplified bioinformatics, and an intuitive workflow that enable the widest range of applications and flexibility of scale on a benchtop sequencing system.NextSeq 550 Instrument NextSeq 550Dx Instrument; Regulatory Status: For Research Use Only. Not for use in diagnostic procedures. For In Vitro Diagnostic Use. Contact us for regional availability. Key Applications: Exome Sequencing; Targeted Gene Sequencing (amplicon, gene panel) Whole-Transcriptome Sequencing; Cytogenomic Arrays; Diagnostic ...Nov 1, 2022 · During this step, the Olink® custom recipe is installed on the NextSeq™ 550. This step only needs to be performed once, before an Olink sequencing run is performed for the first time. NOTE: The Olink custom recipe will only work with the NextSeq™ 500/550 High Output Kits and the NextSeq™ Control Software 4.0. 1. The NextSeq 550 System offers various throughput levels, providing users with optimal operational efficiency. The NextSeq 550 System also delivers a one-day turnaround for numerous popular sequencing applications. With this instrument, users can sequence a broad range of samples per run: • 1-12 exomes • 1-16 transcriptomes NextSeq 550 System Sequencing Performance Parameters. *Install specifications based on Illumina PhiX control library at supported cluster densities (between 129 and 165 k/mm² clusters passing filter). Actual performance parameters may vary based on sample type, sample quality, and clusters passing filter. All NextSeq 550 kits are paired-end ...sequencing systems and flow cells. However, the sequencing throughput has been optimized for NovaSeqTM 6000, NextSeqTM 2000, and NextSeq 550 systems (Table 1). Immuno-reaction Olink Explore accommodates a wide range of samples, including serum, plasma, and cerebrospinal fluid (CSF). Samples are first diluted based on protein abundance andExome sequencing analyzes the protein-coding region of the genome, as a cost-effective alternative to whole-genome sequencing. Exome sequencing with the NextSeq 550 System offers a simple and reliable method to identify coding variants, successfully sequencing even the most difficult genomic regions. Sequence 3–12 exomes per NextSeq run.The CCR Genomics Core provides Next-Generation Sequencing (NGS) services using Illumina sequencing technology. The Core facility has multiple Illumina desktop sequencers to include the iSeq 100, MiSeq, NextSeq 550 and the NextSeq 2000. Each sequencing platform delivers exceptional data quality and performance, with flexible throughput and ... The NextSeq 550Dx System, a Next-Generation Sequencing Instrument with the following specifications: Capable of generating 2 × 36 paired-end reads; Compatible with index adapters in the VeriSeq NIPT Sample Prep …Illumina NextSeq 550 System, funded by a NIH grant (PI, Wang), is an integrated system for automated generation of DNA clonal clusters by bridge amplification, ...... NextSeq 550 system and reagent kits · Large image · Feedback · Image · Back cover. Close Information Start Slideshow. Previous Image. Next Image.NextSeq 500 and 550 System Denature and Dilute Libraries Guide. Instructions for preparing PhiX and denaturing and diluting libraries for sequencing on the NextSeq …NextSeq 500/550 v2.5 sequencing reagent kits deliver the power of high-throughput sequencing on a benchtop system with increased stability and robustness. These kits offer: New v2.5 flow cell provides greater stability and robustness. Continued use of proven v2 reagent and buffer cartridges. Multiple options for both sequencing output and read ...DRAGEN v3.10.11 (planned for release in mid-December 2022) onboard the NextSeq 1000/2000 is recommended to generate FASTQ files with 600 cycle kits. DRAGEN versions before 3.10.11 may experience failures during demultiplexing with 600 cycle runs, requiring requeue of FASTQ generation (detailed in the NextSeq 1000 and 2000 Sequencing …NextSeq 500/550 v2.5 sequencing reagent kits deliver the power of high-throughput sequencing on a benchtop system with increased stability and robustness. These kits offer: New v2.5 flow cell provides greater stability and robustness. Continued use of proven v2 reagent and buffer cartridges. Multiple options for both sequencing output and read ... Barcoded cDNA libraries were sequenced on an Illumina NextSeq 500 instrument using a NextSeq 500/550 High Output Kit v2 (150 cycles) (20024907, Illumina) with the following cycle counts: 28 (read ...Current NextSeq 2000 Customers Access new features through simple customer installable upgrades For Research Use Only. Not for use in diagnostic procedures. 8 NextSeq™ 2000 system (ICS v1.1.0) Custom primers & recipes On board data compression New DRAGEN analysis pipelines New P2 & P3 consumables ICS v1.2.0 DRAGEN v3.7.4NextSeq 500 System Guide in Spanish. PDF (1 MB) Feb 22, 2022. Instructions for using the NextSeq 500 sequencing system.NextSeq 550 System: Samples per run (high output): 16 (8 DNA + 8 RNA), 10 if DNA only, 16 if RNA only: 2 × 101 bp (max recommended) ... NextSeq 500/550 v2.5 Kits. These sequencing reagent kits offer increased stability and robustness over the v2 kit version. TruSight Oncology 500.Library Prep and Array Kit Selector. Determine the best kit for your project type, starting material, and method or application. Local Run Manager Module Selector. Find the analysis modules compatible with your instrument and Local Run Manager configuration. Sequencing Coverage Calculator. Determine reagents and sequencing runs for your …Jan 14, 2020 ... “NextSeq 1000 and 2000 are designed to enable core labs, small to medium research labs and clinical facilities to access high intensity .... SY-415-1002. Illumina NextSeq 550 SequenIllumina NextSeq 550 System Information about the Il The NextSeq 550 offers flexible high-throughput sequencing capacity for exome, transcriptome and whole-genome sequencing of all sizes of genomes, ... data quality enjoyed by NextSeq 550 and MiSeq™ System users. Figure 1: System Instrument NextSeq 500/550 15046626 SY- 415- 1002 NextSeq® 550 Sequencing System Illumina NextSeq 550 Sequencing System is for Research Use Only and is an integrated system for automated generation of DNA clonal clusters by bridge amplification, sequencing, primary analysis, and array scanning. System includes embedded touchscreen The NextSeq 550Dx System, a Next-Generation Sequencing Instrument wi...

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